Human SUMO I, active

(Catalog # P003; P012)

Description:

The active human SUMO I (the 1-97 animo acid region of the Ubiquitin-like protein SMT3C precursor [access no: Q93068, also called Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, Ubiquitin-related protein SUMO-1, GAP modifying protein 1, GMP1, and Sentrin]) is produced from a E. coli expression system, prepared from freshly extracted cell lysates, and purified to homogeneity using methods developed by LAE Biotechnology Co., LTD. The final fraction of enzyme contains a single polypeptide band of 11 kDa.

Concentration:

LAE-SUMO I is supplied at the concentration of 0.5ug/ul (Cat # P003: 100ug/tube, 200ul/tube; Cat # P012: 50ug/tube, 100 ul/tube).

The recommended working concentration is 50ug/ml.

Storage Buffer:

The final fraction of LAE-SUMO I is an affinity column pool and is in the following buffer: 10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol. The enzyme is stable in this buffer at -20~70 ^oC. In general, the enzyme is less stable when diluted. We don’t recommend any dilution of the enzyme.

Shipping/Storage:

The enzyme is shipped on ice and must be stored at -20^oC or lower.

Legal consideration: FOR RESEARCH USE ONLY.

Figure: TOPO I sumoylation with sumo-1 and sumo-3

Right Lane: Topo I protein (TopoI: Cat# B007)

Middle Lane: Sumo-I sumoylated Topo I (Sumo-I: Cat# P003; TopoI: Cat# B007; EI: Cat# P001; EII: Cat# P002; Buffer: Cat# B006)

Left Lane: Sumo-3 sumoylated Topo I (Sumo-3: Cat# P004; TopoI: Cat# B007; EI: Cat# P001; EII: Cat# P002; Buffer: Cat# B006)

Note: Topo I is S³⁵-Met labeled.